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Proteintech
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Proteintech
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Atlas Antibodies
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Proteintech
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R&D Systems
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Journal: Frontiers in Cell and Developmental Biology
Article Title: Garciniagifolone A, derived from Garcinia multiflora fruits, inhibits triple-negative breast cancer cells and organoids growth by targeting CA9
doi: 10.3389/fcell.2026.1767397
Figure Lengend Snippet: GA inhibits TNBC growth via targeting CA9. (a) Venn diagram showing the number of differentially expressed genes in the comparison between MDA-MB-231 and BT549. (b,c) TCGA and GEO databases show that CA9 is highly expressed in TNBC. (d) Correlation analysis between TNBC patient prognosis and CA9 expression. (e) IHC staining of TNBC patient-derived cancer tissue and adjacent tissue. Scale bar: 100 μm. (f) The quantitative analysis of CA9. (g) The protein levels of CA9 in cell lines were measured by Western blotting. (h) The predicted binding mode of GA with CA9. (i) GA binds to CA9 protein as shown by SPR. (j,k) CETSA analysis of CA9 combined with GA. (l,m) CA9 mRNA levels after GA 24 h treatment by qRT-PCR. (n,o) CA9 protein levels after GA 24 h treatment by Western blotting. (p) The related protein levels of siRNA-CA9 TNBC cells after GA 24 h treatment by Western blotting. Data are presented as mean ± SD. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 when compared with control group.
Article Snippet: The
Techniques: Comparison, Expressing, Immunohistochemistry, Derivative Assay, Western Blot, Binding Assay, Quantitative RT-PCR, Control
Journal: Frontiers in Cell and Developmental Biology
Article Title: Garciniagifolone A, derived from Garcinia multiflora fruits, inhibits triple-negative breast cancer cells and organoids growth by targeting CA9
doi: 10.3389/fcell.2026.1767397
Figure Lengend Snippet: Model of the anti-tumor mechanism of GA in TNBC cells. GA could bind CA9 to induce early stage autophagy by blocking PI3K/AKT/mTOR signaling pathway. Moreover, GA suppressed the autophagic flux and increased the p62 to promote ROS production, which activated NLRP3/caspase-1/GSDMD/caspase-3 related pyroptosis and apoptosis to induce TNBC cells death.
Article Snippet: The
Techniques: Blocking Assay